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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
19/09/2014 |
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Actualizado : |
23/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
ALEM, D.; NARANCIO, R.; DIAZ, P.; REBUFFO, M.; ZARZA, R.; DALLA RIZZA, M. |
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Afiliación : |
DIEGO MARTIN ALEM GLISON, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; RAFAEL NARANCIO FERES, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; PAOLA ALEXANDRA DIAZ DELLAVALLE, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MONICA IRENE REBUFFO GFELLER, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; RODRIGO TABARE ZARZA FUENTES, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MARCO DALLA RIZZA VILARO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Molecular characterization of Lotus corniculatus cultivars using transferable microsatellite markers. |
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Fecha de publicación : |
2011 |
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Fuente / Imprenta : |
Ciencia e Investigación Agraria, Santiago de Chile, v. 38, n. 3, p. 463-471, 2011. |
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ISSN : |
0304-5609 |
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DOI : |
10.4067/S0718-16202011000300015 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received September 7 2010. /Accepted June 21, 2011. |
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Contenido : |
Abstract :Lotus corniculatus L. is the most important agricultural species in the genus Lotus and is the most widely distributed Lotus species worldwide. L. corniculatus genotypes form complex groups that are difficult to recognize both morphologically and biochemically. Given the extensive and expensive process of isolating Simple Sequence Repeats (SSR, also called microsatellites), the possibility of using microsatellites already identified in related species is highly attractive. The aim of this work was the identification and validation of transferable microsatellite markers in L. corniculatus, and using those markers to study the genetic variability among four cultivars. Each cultivar of L. corniculatus was represented by 15 genotypes. Ten microsatellite markers were evaluated, and from those, four were selected based on their discriminative values observed among cultivars. We detected 29 alleles for the four markers, and there was an average of 7.25 alleles per locus. The marker TM0197 had the fewest number of alleles (5) and TM0083 had the highest number of alleles (10). The polymorphic information content (PIC) for the selected markers varied from 0.19 to 0.35, and the markers were therefore classified as highly informative. Based on the markers, we found high variability between individuals of the same cultivar. The use of transferable microsatellite markers could be useful to differentiate individuals at a relatively low cost, showing a great potential for use in breeding programs. MenosAbstract :Lotus corniculatus L. is the most important agricultural species in the genus Lotus and is the most widely distributed Lotus species worldwide. L. corniculatus genotypes form complex groups that are difficult to recognize both morphologically and biochemically. Given the extensive and expensive process of isolating Simple Sequence Repeats (SSR, also called microsatellites), the possibility of using microsatellites already identified in related species is highly attractive. The aim of this work was the identification and validation of transferable microsatellite markers in L. corniculatus, and using those markers to study the genetic variability among four cultivars. Each cultivar of L. corniculatus was represented by 15 genotypes. Ten microsatellite markers were evaluated, and from those, four were selected based on their discriminative values observed among cultivars. We detected 29 alleles for the four markers, and there was an average of 7.25 alleles per locus. The marker TM0197 had the fewest number of alleles (5) and TM0083 had the highest number of alleles (10). The polymorphic information content (PIC) for the selected markers varied from 0.19 to 0.35, and the markers were therefore classified as highly informative. Based on the markers, we found high variability between individuals of the same cultivar. The use of transferable microsatellite markers could be useful to differentiate individuals at a relatively low cost, showing a great potential for use in b... Presentar Todo |
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Palabras claves : |
GENETIC VARIABILITY; LOTUS JAPONICUS; MICROSATELLITES; SIMPLE SEQUENCE REPEATS; SSR; VARIABILIDAD GENÉTICA. |
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Thesagro : |
CULTIVOS. |
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Asunto categoría : |
F01 Cultivo |
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URL : |
http://www.scielo.cl/pdf/ciagr/v38n3/art15.pdf
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Marc : |
LEADER 02470naa a2200301 a 4500
001 1050403
005 2019-10-23
008 2011 bl uuuu u00u1 u #d
022 $a0304-5609
024 7 $a10.4067/S0718-16202011000300015$2DOI
100 1 $aALEM, D.
245 $aMolecular characterization of Lotus corniculatus cultivars using transferable microsatellite markers.$h[electronic resource]
260 $c2011
500 $aArticle history: Received September 7 2010. /Accepted June 21, 2011.
520 $aAbstract :Lotus corniculatus L. is the most important agricultural species in the genus Lotus and is the most widely distributed Lotus species worldwide. L. corniculatus genotypes form complex groups that are difficult to recognize both morphologically and biochemically. Given the extensive and expensive process of isolating Simple Sequence Repeats (SSR, also called microsatellites), the possibility of using microsatellites already identified in related species is highly attractive. The aim of this work was the identification and validation of transferable microsatellite markers in L. corniculatus, and using those markers to study the genetic variability among four cultivars. Each cultivar of L. corniculatus was represented by 15 genotypes. Ten microsatellite markers were evaluated, and from those, four were selected based on their discriminative values observed among cultivars. We detected 29 alleles for the four markers, and there was an average of 7.25 alleles per locus. The marker TM0197 had the fewest number of alleles (5) and TM0083 had the highest number of alleles (10). The polymorphic information content (PIC) for the selected markers varied from 0.19 to 0.35, and the markers were therefore classified as highly informative. Based on the markers, we found high variability between individuals of the same cultivar. The use of transferable microsatellite markers could be useful to differentiate individuals at a relatively low cost, showing a great potential for use in breeding programs.
650 $aCULTIVOS
653 $aGENETIC VARIABILITY
653 $aLOTUS JAPONICUS
653 $aMICROSATELLITES
653 $aSIMPLE SEQUENCE REPEATS
653 $aSSR
653 $aVARIABILIDAD GENÉTICA
700 1 $aNARANCIO, R.
700 1 $aDIAZ, P.
700 1 $aREBUFFO, M.
700 1 $aZARZA, R.
700 1 $aDALLA RIZZA, M.
773 $tCiencia e Investigación Agraria, Santiago de Chile$gv. 38, n. 3, p. 463-471, 2011.
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Registro original : |
INIA La Estanzuela (LE) |
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| Registros recuperados : 5 | |
| 2. |  | DIAZ, P.; CABRERA, A.; ALEM, D.; LARRAÑAGA, P.; FERREIRA, F.; DALLA RIZZA, M. Antifungal activity of medicinal plant extracts against phytopathogenic fungus Alternaria spp. [Actividad antifúngica de extractos de plantas medicinales contra el hongo fitopatógeno alternaria spp.] Chilean Journal of Agricultural Research, 2011, v.71, no.2, p.231-239. doi: http://dx.doi.org/10.4067/S0718-58392011000200008 2-s2.0-79960343579 RESEARCH. Received: 2 August 2010. Accepted: 26 December 2010.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : B - 3 |
| Biblioteca(s): INIA Las Brujas. |
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| 3. | | ALEM, D.; NARANCIO, R.; DIAZ, P.; REBUFFO, M.; ZARZA, R.; DALLA RIZZA, M. Molecular characterization of Lotus corniculatus cultivars using transferable microsatellite markers. Ciencia e Investigación Agraria, Santiago de Chile, v. 38, n. 3, p. 463-471, 2011. Article history: Received September 7 2010. /Accepted June 21, 2011.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : B - 3 |
| Biblioteca(s): INIA La Estanzuela. |
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| 4. | | LARRAÑAGA, P.; DIAZ, P.; CABRERA, A.; ALEM, D.; ALMEIDA SOUZA, A.L.; GIOVANNI DE SIMONE, S.; DALLA RIZZA, M. Activity of naturally derived antimicrobial peptides against filamentous fungi relevant for agriculture . Sustainable Agriculture Research, 2012, v. 1, no.2, p. 211-221. Article history: Received: June 12, 2012 / Accepted: July 10, 2012 / Online Published: July 23, 2012.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : C - 0 |
| Biblioteca(s): INIA Las Brujas. |
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| 5. | | ALEM, D.; DIAZ, P.; LEONI, C.; DE SIMONE, S.G.; CORREA, A.; OPPEZZO, P.; DALLA RIZZA, M. In Search of Topical Agricultural Biofungicides: Properties of the Recombinant Antimicrobial Peptide TrxAq-AMP obtained from Amaranthus quitensis. Journal of Microbial & Biochemical Technology, 2014, v. 6, no.5, p. 268-273.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : B - 4 |
| Biblioteca(s): INIA Las Brujas. |
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| Registros recuperados : 5 | |
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